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What can cause poor 16S amplification other than DSS inhibition even after getting good concentration of startingDNA?

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Complex sequence organization of heterochromatin in the holocentric plant Cuscuta europaea elucidated by the computational analysis of nanopore reads - Computational and Structural Biotechnology Journal

Comparison of destructive and nondestructive DNA extraction methods for the metabarcoding of arthropod bulk samples - Kirse - 2023 - Molecular Ecology Resources - Wiley Online Library

Environmental DNA analysis confirms extant populations of the cryptic Irwin's turtle within its historical range, BMC Ecology and Evolution

Frontiers De novo genome and transcriptome assembly of Kelletia kelletii, a coastal gastropod and fisheries species exhibiting a northern range expansion